TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or relevant phenotypea | Source or reference |
|---|---|---|
| Strains | ||
| P. mirabilis | ||
| Wild type | Wild-type (N2); Tetr | Clinical isolate |
| rseA mutant | Wild type derivative; rseA knockout mutant; Kmr | This study |
| rpoE mutant | Wild typederivative; rpoE knockout mutant; Kmr | This study |
| rseAc | rseA mutant strain containing pGEM-T Easy-rseA; RseA-complemented strain; Ampr | This study |
| rpoEc | rpoE mutant strain containing pGEM-T Easy-rpoE; RpoE-complemented strain; Ampr | This study |
| E. coli | ||
| DH5α | fhuA2 lacΔU169 phoA glnV44 W80′ lacZ ΔM15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 | Invitrogen |
| S17-1 λpir | λpir lysogen of S17-1 [thi pro hsdR2 hsdM+ recA RP4 2-Tc::Mu-Km::Tn 7(Tpr Smr)]; permissive host able to transfer suicide plasmids requiring the Pir protein by conjugation to recipient cells | 24 |
| Plasmids | ||
| pGEM-T Easy | High-copy TA cloning vector; Ampr | Promega |
| pUT/mini-Tn5-Km | Suicide plasmid requiring the Pir protein for replication and containing a mini-Tn5 cassette containing the Kmr gene | 24 |
| pACYC184 | Low-copy cloning vector, P15A replicon; Cmr Tetr | 24 |
| pGEM-T Easy-rseA | pGEM-T Easy containing intact rseA sequence, including its ribosome binding site (RBS); Ampr | This study |
| pGEM-T Easy-rpoE | pGEM-T Easy containing intact rpoE sequence, including its ribosome binding site (RBS); Ampr | This study |
| pACYC184-rpoE- xylE | rpoE reporter plasmid, pACYC184 containing intact rpoE promoter sequence before xylE; Cmr | 24 |
a
Cmr, chloramphenicol resistance; Tetr, tetracycline resistance; Ampr, ampicillin resistance; Smr, streptomycin resistance; Kmr, kanamycin resistance; Tpr, trimethoprim resistance.