FIG 1.

Effects of deletion of acuK and acuM on growth on gluconeogenic substrates and under iron-limited conditions. (A) Serial 10-fold dilutions of the indicated strains were plated on agar containing glucose, ethanol, acetate, or proline as the carbon source. The plates were imaged after incubation at 37°C for 48 h. (B) Dry weight of the indicated strains after 48 h of growth at 37°C in Sabouraud dextrose broth containing either 30 mM phenanthroline plus 1 mM FeCl₂ (+Iron) or phenanthroline without FeCl₂ (−Iron). Results are means ± SDs from 3 independent experiments, each performed in triplicate. *, P < 0.0001 compared to the wild-type strain (WT) grown under iron limitation; †, P < 0.0001 compared to the ΔacuK+acuK complemented strain grown under iron limitation.