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. 2015 Feb 13;83(3):1056–1067. doi: 10.1128/IAI.02725-14

FIG 6.

FIG 6

Exponentially growing cells are susceptible to serum. (A) Cells were grown to exponential phase in LB medium and diluted in 50% HIS or 50% serum. Cell division profiles of exponential-phase cells were determined at the start and 60 and 120 min after dilution. The relative number of events with particular GFP fluorescence levels in the same culture volume is shown. (B) Cells were grown to stationary (Stats) or exponential (Exp) phase in LB medium and diluted in 50% HIS or serum. To inhibit cell growth, chloramphenicol (Cam; 50 μg/ml) was added to one parallel sample. The CFU were counted after the indicated time by plating, and the percentage of surviving cells compared to the zero time point is shown. The averages and standard deviations of at least three independent experiments are presented.