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. 2015 Feb 13;83(3):986–995. doi: 10.1128/IAI.02955-14

FIG 3.

FIG 3

Killing of C. difficile by α-defensins results in a peculiar pattern of damage. (A) Dot blots of C. difficile CD630 (RT012), E. coli ATCC 25922, and E. faecalis ATCC 29212 populations after incubation with 7 μM HD5, HNP1, or control peptide RL26495. Membrane depolarization and cell size were recorded by FACS analysis. (B) Quantification of dose-dependent bacterial damage. Percentages of undamaged bacteria (gate A), depolarized fluorescent bacteria (gate B), and bacterial cell fragments (gate C) were obtained by treatment with increasing concentrations of α-defensins (open symbols) or control peptide RL26495 (solid symbols) and analyzed by FACS. Circles, CD630; squares, E. coli ATCC 25922; triangles, E. faecalis ATCC 29212. Each data point is representative of 4 to 6 independent experiments. Error bars show mean values ± SEMs. *, P < 0.05; ***, P < 0.001 (compared to C. difficile) according to Fisher's exact test.