FIG 5.

IL-12β cytokine response to in vivo stimulation of lamina propria lymphocytes (LPLs) by V. cholerae. LPLs were cultured from duodenal biopsy specimens taken from cholera patients in the acute (day 2, D2) and convalescent (day 30, D30) stages and from healthy control (HC) patients. IL-12β was measured in cell culture supernatants (pg/ml). LPLs isolated from acute-stage biopsy specimens produced significantly more IL-12β than those taken from convalescent-stage biopsy specimens, as determined by Student's t test (P < 0.05). LPLs isolated from four of the five healthy biopsy specimens did not produce any detectable IL-12β.