FIG 1.
5-LO is induced by B. abortus infection in 129 Sv/Ev mice and downregulates host resistance to this pathogen. 129 Sv/Ev and 5-LO KO mice were infected with B. abortus 2308 (1 × 106 CFU/mouse). (A) Splenocytes were obtained 1 week after infection, and the relative expression of 5-LO mRNA was analyzed by RT-PCR. Uninfected mice were used as controls. (B and C) The bacterial load was analyzed in the spleens of mice (1, 2, 3, and 6 weeks postinfection) and in livers (1, 3, and 6 weeks postinfection) by CFU counting. Results are expressed as means ± standard deviations and are representative of 3 independent experiments (n = 5). *, P ≤ 0.05 compared to 5-LO KO infected mice; &, P ≤ 0.05 compared to 129 Sv/Ev uninfected mice.