FIG 5.

ElpA alters host secretory transport. (A) HEK293T cells were cotransfected with a SEAP-expressing construct and GFP alone or one of the indicated GFP fusion proteins. SEAP levels were determined and are displayed as the ratio of intracellular to extracellular product. Experiments were performed in triplicate, and error bars represent the standard deviations from the means. *, P < 0.005; n.s., not significant (Student's t test). All GFP-ElpA samples were compared to GFP alone. The N- and C-terminal portions of ElpA alone do not significantly alter secretion, but full-length ElpA triggers approximately 60% less secretion than the GFP-only control, indicating that ElpA negatively influences host secretory transport.