TABLE 1.
Bacteria and plasmids used in this study
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| Strains | ||
| C. burnetii | ||
| Dugway (7E65-68) | Phase I, Utah, rodent, 1958 | 36 |
| Nine Mile (RSA493) | Phase I, Montana, tick, 1936 | 37 |
| L. pneumophila | ||
| JR32 | Salt-sensitive isolate of AM511 | 38 |
| LELA3118 | JR32 dotA::Tn903dIIlacZ3118 DotA− Kmr | 38 |
| E. coli TOP10 | F− mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| Plasmids | ||
| pJB2581 | cyaA fusion vector, Cmr | 39 |
| pDHVAnkN | ankN in pJB2581 | 5 |
| pDHVElpA1–343 | elpA N-terminal fragment in pJB2581 | This study |
| pDHVElpA341–418 | elpA C-terminal fragment in pJB2581 | This study |
| pCR2.1-TOPO | TA TOPO vector, Ampr | This study |
| pENTR-D/TOPO | Gateway entry vector, Kmr | This study |
| pcDNA6.2/N-EmGFP | N-terminal EmGFP fusion vector, Ampr | This study |
| GFP-ElpA | EmGFP::elpA | This study |
| GFP-ElpA1–343 | EmGFP::elpA N-terminal fragment | This study |
| GFP-ElpA-C341–418 | EmGFP::elpA C-terminal fragment | This study |