TABLE 2.
Primers used in this study
| Purpose and primer | Sequencea |
|---|---|
| pJB2581 cloning | |
| ElpA-N-F | CATGCGGATCCGTGAGTCGAATAATTTATGATGAG |
| ElpA-N-R | CGCATGCGTCGACTTAATCAATAACGGGTGCTCC |
| ElpA-C-F | CATGCGGATCCTTGATTAATCTTCAGGAGGG |
| ElpA-C-R | CGCATGCGTCGACTTAAAAGTTAAATCTGGGGGC |
| pENTR cloning | |
| GFP-ElpA-N-F | CACCATGGGCGTGAGTCGAATAATTTATGATGAG |
| GFP-ElpA-N-R | TTAATCAATAACGGGTGCTCC |
| GFP-ElpA-C-F | CACCATGGGCTTGATTAATCTTCAGGAGGGTAATAATAG |
| GFP-ElpA-C-R | TTAAAAGTTAAATCTGGGGGCTTCTTC |
| RT-PCR | |
| ElpA-F | GAAACGAGAGAGAAGAAGAGGAAG |
| ElpA-R | GGTGTCTATTATTACCCTCCTGAAG |
| ElpA-probe | AAGATCCGGAGCACCCGTTATTGA |
a
Underlining indicates restriction sites.