FIG 2.

ME mutants are deficient in malate catabolism. (A) WT bacteria were tested for malate utilization by measuring growth over the course of 16 h of cultures grown in either unmodified C medium or C medium supplemented with 0.5% malate. Data are presented as means and standard deviations from 3 independent experiments. (B and D) WT and ME mutants were grown in unmodified C medium or C medium supplemented with 0.5% malate. Following 16 h of incubation, growth yields were measured by OD₆₀₀. Data are presented as the means and standard deviations from 3 independent experiments. (C and E) Malate concentrations from cell-free culture supernatants from overnight cultures of WT or ME mutants grown in C medium supplemented with 0.5% malate were measured (see Materials and Methods). Data are presented as percent remaining (compared to initial concentration) and are presented as means and standard deviations from 3 biological samples analyzed in duplicate. Asterisks indicate significant differences (***, P < 0.001) compared to WT in C medium.