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Cryo sections through 24-h biofilms from NTHI 2019 wild type (A), NTHI 2019Δnuc (B), and NTHI 2019Δnuc::nuc (C). The DNA matrix is stained with DAPI, and the NTHI strains are stained with MAb 6E4. Scale bar, 20 μm. The organisms in the parent strain and in the complemented mutant are clearly dispersed throughout the biofilm, whereas they are clustered in the Δnuc mutant.
