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Lateral views and stacked z-series of 48-h biofilms stained with Live/Dead stain (green-red) and DRAQ-5 (blue). (A and C) Biofilm images of NTHI 2019 demonstrating a predominance of live organisms (green), while the images of NTHI 2019Δnuc biofilm (B and D) demonstrate that the majority of organisms were dead (red) by 48 h.
