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. 2015 Feb 13;83(3):950–957. doi: 10.1128/IAI.02601-14

TABLE 1.

Bacterial strains, plasmids, and primers used in this study

Nontypeable H. influenzae strain or plasmid Genotype, relevant characteristic(s), and/or sequence (5′-3′)a Source or reference
NTHI strains
    2019 Wild type Our laboratory
    2019Δnuc Δnuc; Kan This study
    2019Δnuc::nuc Δnuc::nuc; Kan Sp This study
Plasmids
    pGEM-T Promega
    pET151/D-TOPO Amp Life Technologies
    pUC18K3 Kan This laboratory
    p601.1-Sp2 Sp 10
    pCEC#27 pUC18K3::Δnuc::kan; Kan This study
    pCEC#29 pET151::nuc; Amp This study
    pCEC#31 p601.1Sp2::nuc; Sp This study
Primers
    DNase 1 FRET assay primer Cy3-CCGCGAGAAACCAAGCACAGAGCACCGAAGA-BHQ_2
    qRT-PCR primers
    nuc primers
    Forward AGGGTATGGCATGGGCTTAT
    Reverse GTGCGAGCCTGTTCATATTG
    OMP p6 primers
    Forward GGTATTCFTGGGTGGTTGCGTATT
    Reverse AGATCAGCAACAGAGTAACCGCCA
a

Kan, kanamycin; Sp, spectinomycin; Amp, ampicillin; BHQ, black hole quencher.