TABLE 1.
Nontypeable H. influenzae strain or plasmid | Genotype, relevant characteristic(s), and/or sequence (5′-3′)a | Source or reference |
---|---|---|
NTHI strains | ||
2019 | Wild type | Our laboratory |
2019Δnuc | Δnuc; Kan | This study |
2019Δnuc::nuc | Δnuc::nuc; Kan Sp | This study |
Plasmids | ||
pGEM-T | Promega | |
pET151/D-TOPO | Amp | Life Technologies |
pUC18K3 | Kan | This laboratory |
p601.1-Sp2 | Sp | 10 |
pCEC#27 | pUC18K3::Δnuc::kan; Kan | This study |
pCEC#29 | pET151::nuc; Amp | This study |
pCEC#31 | p601.1Sp2::nuc; Sp | This study |
Primers | ||
DNase 1 FRET assay primer | Cy3-CCGCGAGAAACCAAGCACAGAGCACCGAAGA-BHQ_2 | |
qRT-PCR primers | ||
nuc primers | ||
Forward | AGGGTATGGCATGGGCTTAT | |
Reverse | GTGCGAGCCTGTTCATATTG | |
OMP p6 primers | ||
Forward | GGTATTCFTGGGTGGTTGCGTATT | |
Reverse | AGATCAGCAACAGAGTAACCGCCA |
Kan, kanamycin; Sp, spectinomycin; Amp, ampicillin; BHQ, black hole quencher.