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. 2015 Jan 27;112(4):693–703. doi: 10.1038/bjc.2015.16

Figure 4.

Figure 4

LiCl and BIO reduce migration of pHGG cells in an aligned nanofibre 3D migration assay. Tumour cell aggregates formed from hanging drops and labelled with a fluorescent cell tracker were placed in wells containing aligned poly-ɛ-caprolactone fibres and cultured±20 mM LiCl or 5 μM BIO. Migration was recorded either as phase contrast or fluorescent images for quantification. (A) Spheroids formed from cell line SF188 migrated along the nanofibre as evidenced by phase contrast and fluorescent imaging; the addition of LiCl or BIO had a marked anti-migratory effect on the cells. Images at × 40 magnification. Scale bar=400 μm. (B) Quantification and comparison of the effects of 20 mM LiCl and 5 μM BIO on the migration index for both cell lines. Results representative of at least three repeats. Error bars are expressed as ±s.e.m. *P<0.05, **P<0.01, ***P<0.001 by ANOVA for each cell type and treatment. (C) Quantification and comparison of the effects of 20 mM LiCl and 5 μM BIO on the perimeter index for both cell lines. Error bars are expressed as ±s.e.m. *P<0.05, **P<0.01, ***P<0.001 by ANOVA for each cell type and treatment.