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. 2015 Jan 27;112(4):739–744. doi: 10.1038/bjc.2015.1

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Copyright © 2015 Cancer Research UK

From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

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Downregulation of HtrA2 by a specific siRNA decreased the sensitivity to cisplatin in cisplatin-sensitive serous ovarian cancer cells. (A) Downregulation of HtrA2 by a specific siRNA was observed in KF28 cells by western blotting analysis. (B) HtrA2 protein expression level of KF28 cells transfected with non-specific control siRNA (KF28-C), and HtrA2-specific siRNA (KF28-H) were analysed. Blots were scanned and quantified by densitometry. HtrA2 expression level of KF28-H was significantly lower than that of KF28-C. *P<0.01 (C) Cell viability was evaluated by the MTT assay. Five days after transfection with siRNA, sensitivity to cisplatin was measured by 24-h cisplatin treatment, and KF28-H showed resistance to cisplatin treatment. The data represented the mean±s.d. of at least three experiments. *P<0.01.