Figure 4.

Eutectic 5′ addition of other substrates activated by 2′,3′-cyclic phosphates. (a) Transfer of the trinucleotide ApCpA>p (72 h, −7 °C). (b) Time series of multiple GpUpCpCpA>p (25 μM) 5′-additions by a HPz derivative. Small amounts of transfer did already occur during sample preparation (*). The left lane shows a primer-only control. (c) Transfer of NAD>p at different dinucleotide concentrations (96 h, −7 °C).