Table 1. LWV Shift Values of Protein–Small Molecule Binding Interaction Detected on the ECL Biosensor^a.

	CA IIb	NQO1c	XIAPd	hLDH-Ae	Casp-3f
dorzolamide	12.5 ± (1.1)	–2.4	1.5	2.6	0.3
dicoumarol	1.2	11.2 ± (1.0)	–1.1	–1.5	–0.5
SM-164	–0.9	0.7	11.5 ± (0.5)	–0.2	1.4
NHI-1	1.8	2.0	1.8	14.0 ± (1.0)	1.2
QVD-OPh	–1.5	1.1	–0.2	0.4	19.3 ± (1.9)

^aLWV shift values are reported in pm. Values in bold represent the mean of at least 3 independent measurements for the five cognate protein–small molecule pairs with positive binding signal. Values in parentheses indicate standard error of mean. Binding tests were performed using 50 μM of small molecule solution with 40 μg of immobilized protein at RT.

^bCA II, carbonic anhydrase isozyme II.

^cNQO1, human NAD(P)H dehydrogenase quinone 1.

^dXIAP, X-linked inhibitor of apoptosis protein.

^ehLDH-A, human lactate dehydrogenase-A.

^fCasp-3, caspase-3.