Table 1. DLS z-Average Diameter (D_z) and Polydispersity Index (PDI) and Various SAXS Structural Parameters Obtained by Analysis of a Series of G₅₅-H_x Copolymer Vesicles^a.

DLS			SAXS									CD-MS
PHPMA DP (x)	Dz, nm	PDI	Dv, nm	Vm, nm3	RmSAXS, nm	σRm, nm	TmSAXS, nm	σTm, nm	Rg, nm	xsol	NaggSAXS/103	MnCD-MS, MDa	MwCD-MS/Mn	NaggCD-MS /103	⟨z⟩ / e	D, nm	TmCD-MS, nm
200	239	0.28	244(5)	40(4)	111(3)	45(2)	12.8(1)	1.3(1)	2.2(1)	0.38(1)	31.0 ± 5.3	–	–	–	–	–	–
			233(7)		105(3)	50(2)	12.6(1)	1.1(1)	2.5(3)	0.51(1)	22.0 ± 3.9
300	219	0.14	236(6)	59(6)	105(3)	45(1)	17.8(1)	2.1(1)	2.1(1)	0.38(1)	25.7 ± 4.7	800	1.19	15.3	1952	177	12.6
			228(6)		101(4)	51(2)	17.8(1)	2.1(1)	2.4(1)	0.52(1)	18.2 ± 3.8
400	201	0.12	208(4)	79(9)	89(2)	39(1)	20.2(1)	2.2(1)	2.2(1)	0.45(1)	14.2 ± 2.5	1307	1.18	13.5	2076	184	20.8
			213(3)		91(1)	45(1)	20.3(1)	2.3(1)	2.4(1)	0.56(1)	11.8 ± 1.9
500	208	0.02	197(2)	99(11)	82(1)	34(1)	24.7(1)	2.3(1)	2.3(1)	0.38(2)	13.0 ± 2.0	1023	1.09	12.6	1838	170	19.2
			197 (2)		81(1)	36(1)	25.1(1)	2.6(1)	2.4(1)	0.52(2)	10.2 ± 1.8
600	204	0.04	205(2)	119(13)	83(1)	38(1)	29.7(1)	2.7(1)	2.3(1)	0.51(2)	10.8 ± 1.8	1366	1.12	14.3	2060	184	22.6
			206(1)		83(1)	40(1)	30.4(1)	3.0(1)	2.3(1)	0.58(2)	9.5 ± 1.8
700	204	0.04	205(2)	139(15)	82(1)	34(1)	34.1(1)	3.0(1)	1.7(1)	0.63(1)	7.9 ± 1.3	1421	1.08	12.9	2181	190	21.4
			205(2)		82(2)	40(1)	34.5(1)	3.0(1)	1.8(1)	0.63(1)	7.9 ± 1.3
800(A)	205	0.02	204(3)	158(17)	79(1)	32(1)	37.7(1)	2.9(1)	2.0(1)	0.62(1)	7.3 ± 1.2	1282	1.11	10.3	2046	183	21.1
			206(3)		80(2)	35(1)	38.9(1)	3.1(1)	1.9(1)	0.61(2)	7.9 ± 1.5
800(B)	201	0.03	197(1)	158(17)	75(1)	30(1)	37.1(1)	4.8(1)	2.3(4)	0.57(1)	7.4 ± 1.1	1220	1.11	9.8	2000	180	20.7
			194(1)		73(4)	32(1)	39.1(1)	5.3(1)	2.3(1)	0.62(1)	6.4 ± 0.8
1000	229	0.02	243(3)	198(21)	95(2)	41 (1)	45.7(1)	3.8(1)	1.7(1)	0.68(1)	8.4 ± 1.6	2003	1.15	17.9	2413	204	27.8
			247(3)		97(2)	52 (1)	46.9(1)	3.9(1)	1.6(1)	0.66(1)	9.7 ± 1.6
1500	455	0.23	–	–	–	–	–	–	–	–	–	4027	1.21	19.1	3070	240	45.6
2000	752	0.14	–	–	–	–	–	–	–	–	–	97/35700b	n/a	0.362/125.2	–	–	–

^aD_v is the mean vesicle diameter, R_m^SAXS is the radius from the center of the vesicle to the center of the membrane and σ_Rm is the associated standard deviation, T_m is the membrane thickness, and σ_Tm is the associated standard deviation, R_g is the radius of gyration of the coronal stabilizer (PGMA) chains, x_sol is the average water content of the hydrophobic PHPMA membrane, and N_agg^SAXS is the mean vesicle aggregation number. Numbers in brackets represent the error in the last significant digit of the SAXS fitting parameters. The volume of a PGMA₅₅ block (V_vc) was calculated to be 11.2 ± 0.12 nm³ and V_m is the volume of the hydrophobic PHPMA_x block. Two sets of SAXS fitting parameters are given for each sample: the first line corresponds to a ‘simple’ model fit, and the second line corresponds to a ‘complex’ model fit that accounts for the vesicle corona scattering length density profile. CD-MS results obtained for the same series of copolymer vesicles are also summarized, including the number-average particle mass of the vesicles (M_n), weight-average particle mass of the vesicles (M_w^CD-MS), polydispersity index (M_w/M_n^CD-MS), mean vesicle aggregation number (N_agg), average number of charges per vesicle (⟨z⟩; as calculated from the charge distribution), equivalent vesicle diameter (D) and vesicle membrane thickness (T_m^CD-MS); see main text for details.

^bA bimodal mass distribution was observed for this particular sample.