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. 2014 Sep 16;24(5):663–676. doi: 10.1089/scd.2014.0343

FIG. 3.

FIG. 3.

Time course of hypoxia-mediated enhancement of AA-induced expression of Foxa2. (A) mESCs were cultured under hypoxia in the SP media or 20 ng/mL AA media for up to 10 days. At the indicated time points, relative expression of Foxa2 was measured using qPCR. The data are presented as fold of the maintenance culture (mean±SEM, n=5–11). (B) Schematic presentation of the experimental protocol to examine the role of the duration of hypoxia on endoderm differentiation. White bars: duration of normoxia; gray bars: duration of hypoxia. (C) mESCs were cultured in normoxia/hypoxia according to the scheme depicted in (B) in 20 ng/mL AA-containing media for a total of 6 days. Relative expression of Foxa2 was measured by qPCR for all experimental setups at day 6 in culture. The data are presented as fold of maintenance culture (mean±SEM, n=5–14, *P<0.05 vs. 0 day in hypoxia).