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Quality control during airway patch generation. Hematoxylin and eosin staining of the apical (A) and basal region (B) of the decellularized scaffold reveals the extracellular matrix of the former jejunum without cellular residues. The MTT assay shows successful re-endothelialization with microvascular endothelial cells (C) and reseeding with skeletal muscle cells (D). Arrows in (C, D) exemplarily point to MTT-positive areas. A, artery; V, vein.
