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. 2015 Feb 18;11(2):e1004680. doi: 10.1371/journal.ppat.1004680

Fig 3. Ded1p depletion leads to increased level of TBSV minus-strand synthesis on recRNAs and degRNAs in yeast.

Fig 3

(A) Top and bottom panels: Northern blot analysis of (+) and (-)RNA levels, respectively, in TET::DED1 yeast after depletion of Ded1p level (+dox samples) or normal Ded1p level (-dox, no doxycyclin added).The ratios of the most abundant recRNAs versus repRNA (DI-AU-FP) and degRNA versus repRNA were calculated. See further details in Fig. 1. (B) Similar to panel A, except TET::DED1 yeast was grown at 23°C. (C) Similar to panel A, except DI-72 repRNA was expressed in TET::DED1 yeast for TBSV recombination studies. Note that DI-72 repRNA is a much better template than the longer DI-AU-FP repRNA. Also, the recRNAs are larger than DI-72 repRNA, as indicated by arrowheads.