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. 2015 Feb 18;11(2):e1004680. doi: 10.1371/journal.ppat.1004680

Fig 8. Opposite effects of subverted cellular DEAD-box helicases on TBSV recombination in plants.

Fig 8

(A) Over-expression of AtRH2 or AtRH20 was done in N. benthamiana leaves by agroinfiltration. The same leaves were agro-infiltrated to co-express CNV helper virus and the DI-AU-FP repRNA from the 35S promoter. The control samples were obtained from leaves not expressing AtRH2 or AtRH20 proteins (lanes 5–8). Total RNA was extracted from leaves 4 days after agroinfiltration. The accumulation of repRNA, recRNAs and degRNA in N. benthamiana leaves was measured by Northern blotting (top panel), see Fig. 1 for details. The ribosomal RNA (rRNA) was used as a loading control and shown in agarose gel stained with ethidium-bromide (bottom panel). (B) Over-expression of AtRH20 inhibits recRNA formation from the degRNAs. AtRH20 was over-expressed in N. benthamiana leaves by agroinfiltration. The same leaves were co-agro-infiltrated to co-express CNV p33 and p92 replication proteins and the DI-RIΔ degRNA from the 35S promoter. The control samples were obtained from leaves not expressing AtRH20 protein (lanes 1–3). See further details in panel A.