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. Author manuscript; available in PMC: 2015 Feb 19.
Published in final edited form as: J Cell Sci. 2013 Feb 26;126(0 8):1753–1762. doi: 10.1242/jcs.115246

Fig. 3. Wild-type and ΔK32 lamin A bind LAP2α in vitro and in vivo.

Fig. 3

(A) Ponceau protein staining of bacterially expressed and blotted recombinant wild-type prelamin A, ΔK32-prelamin A, or vimentin on nitrocellulose, and autoradiogram of the same blot after probing with [35S] labelled LAP2α are shown. (B) Autoradiography of in vitro-translated [35S} labelled LAP2α separated by SDS-PAGE. (C) Immunoblots of total cell lysates of ΔK32-lamin A/C fibroblasts (5% of input) and immunoprecipitates obtained with lamin A/C antibodies or empty beads (mock).