Table 1.
Gene specific primers used in qRT-PCR experiments
| Gene | Primers | Temperature |
|---|---|---|
| β-actin | Fw: GCTTCACCACCACAGCCGAAAGA; | 60°C/63°C |
| Rev: GATACCGCAAGATTCCATACCCAGG | ||
| lef1 | Fw: AAGGCCACCCGTACCCGAGT; | 60°C |
| Rev: GGGTGAACGGCATGGGACGG | ||
| pea3 | Fw: GCCTGGCTGCCCATCCATGT; | 63°C |
| Rev: AATTCCATGCCACGGCCCGT | ||
| wnt5b | Fw: TTGACGGACAAGCTGTTCAACCAA; | 63°C |
| Rev: ACCACCACGAGTTGGCGACC | ||
| krt8 | Fw: CGGTCTTGGCATGGGCATGGG; | 63°C |
| Rev: TGGAGCGTGTGGCTGTCTGGT | ||
| fgf24 | Fw: CGCCACTTACTGGAGCGGCAA; | 63°C |
| Rev: GGCTCACGTCGTCTCGAGTG | ||
| msxb | Fw: CCAGCAGGTCGCGTGTTCTCC; | 60°C |
| Rev: GCTTGCGTAAGGTGCACGGC | ||
| msxc | Fw: AGGGACAGTCCGGCTGGTTTCA; | 60°C |
| Rev: ACTGCGAGGTGGTAAACGGGG | ||
| mkp3 | Fw: GGTTCGCGCGGAGATGCAAGA; | 63°C |
| Rev: CCCTCCGAGACCCAGGACCTG | ||
| fgf20a | Fw: GGTTCGGTCCAAGGCACGAGG; | 63°C |
| Rev: CGCTCGCCATGCCGATACAGG | ||
| fgfr1 | Fw: ACACGCCTGCGCAACGATCA; | 63°C |
| Rev: GTTGAGCCCAGACGGGTGCC | ||
| mps1 | Fw: ACCAGTAGGGAGCACGCGCT; | 63°C |
| Rev: GGCAGGTGTCCGGGGAGTTTG | ||
| shh | Fw: GGCCAGGGGTTAAGCTGCGT; | 63°C |
| Rev: CGGCCTTCTGTCCTCCGTCC | ||
| ptc1 | Fw: TCTGCAAGCCACTTTTGATG | 63°C |
| Rev: AGGATGGGGGTAAAAGTTGG |