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BIX01294 repressed the production of RANKL-induced osteoclast differentiation markers. Raw264.7 cells were treated with mouse recombinant RANKL (50 ng/mL) for 72 h in the absence or presence of indicated concentrations of BIX01294. The cells were lysed in lysis buffer, and 10 μg of total protein was analyzed by western blotting. Glyceraldehyde-3-phospate dehydrogenase (GAPDH) was used as the internal control. BIX, treated with BIX01294. The arrowheads indicate desired bands.
