FIGURE 4.

BIX01294 decreased RANKL-induced NFATcl production. Raw264.7 cells were treated with mouse recombinant RANKL (50 ng/mL) for 24 h in the absence or presence of the indicated concentrations of BIX01294. The cells were lysed in lysis buffer, and 4 μg of total protein was analyzed by western blotting. GAPDH was used as the internal control. BIX, treated with BIX01294. The arrowheads indicate desired bands.