Fig. 5.

IFNγ co-culture ELISpot using CKC infected with recombinant MVA and splenocytes from birds challenged with LPAI. ELISpot responses in splenocytes from infected birds from unvaccinated (PBS group, n = 3) or birds vaccinated with a recombinant vaccine Fowlpox (F9 group, n = 4), or Fowlpox^NpM1 (F9-NpM1 group, n = 4). Splenocytes were cultured with either irradiated MVA^GFP infected CKC (black dot) or irradiated MVA^NpM1 (white circle) infected CKC. Lines indicate samples from the same spleen under the two previous conditions. Results were expressed as spot forming units (SFU) per million cells. Significance of the data is represented as follows: * = p < 0.05.