Table 3.
Brief details of bioassays contributed to the study.
| laboratory Code | Bioassay cell line** | Assay type | Assay duration (h) | Assay readout |
|---|---|---|---|---|
| 1 | MNFS-60 | Proliferation | 24 | Luminescence (cell-titer Glo) |
| 2 | NFS-60 | Proliferation | 34–38 | Colorimetric (MTS) |
| 3 | G- CSFRLuc | Reporter-gene | 3–4 | Luminescence (luciferase) |
| 4 | NFS-60 | Proliferation | 26–30 | Fluorescence (Alamar Blue) |
| 5 | NFS-60 | Proliferation | 40–48 | Colorimetric (MTT) |
| 6 | GNFS-60 | Proliferation | 48 | 3H Thymidine |
| 7 | NFS-60 | Proliferation | 48 | Colorimetric (MTS) |
| 8 | NFS-60 | Proliferation | 48 | Colorimetric (Cell Titer96 Aqueous One, MTS) |
| 9 | MNFS-60 | Proliferation | 44 | Colorimetric (WST-1) |
| 10 | MNFS-60 | Proliferation | 44–48 | Colorimetric (Cell Titer96 Aqueous One, MTS) |
| 11 | MNFS-60 | Proliferation | 40–44 | Fluorescence (Alamar Blue) |
| 12 | MNFS-60 | Proliferation | 44 | Colorimetric (MTS) |
| 13 | MNFS-60 | Proliferation | 42–44 | Fluorescence (Alamar Blue) |
| 14 | MNFS-60 | Proliferation | 48 | Colorimetric (MTS) |
| 15 | MNFS-60 | Proliferation | 44 | Luminescence (Cell-titer Glo) |
| 16 | NFS-60 | Proliferation | 20–22 | Colorimetric (MTT) |
| 18 | NFS-60 | Proliferation | 48 | Colorimetric (MTS) |
| 19 | MNFS-60 | Proliferation | 44 | Colorimetric (MTS) |
| 20 | MNFS-60 | Proliferation | 28–32 | Luminescence (Cell-titer Glo) |
| 21 | NFS-60 | Proliferation | 48 | Colorimetric (MTT) |
| 22 | NFS-60 | Proliferation | 48 | Colorimetric (MTT) |
| 23 | MNFS-60 | Proliferation | 44 | Colorimetric (MTS) |
| 24 | MNFS-60 | Proliferation | 48 | Colorimetric (Cell Titer96 Aqueous One, MTS) |