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. 2014 Sep 12;21(3-4):426–437. doi: 10.1089/ten.tea.2014.0208

FIG. 2.

FIG. 2.

AD-MSC secreted factors can promote SMC migration. SMCs are induced to migrate through a scratch wound assay while being stimulated with AD-MSC conditioned media over the course of 24 h. AD-MSCs promote the migration of SMCs compared to nonconditioned controls (A). These data were quantified by measuring the normalized wound area over time. These data were converted to migration rate per hour relative to controls, expressing a significant difference between AD-MSC conditioned media and nonconditioned controls (B). In addition, heat inactivating conditioned media caused a significant loss in functionality, indicating that SMC promigratory effects happen at least, in part, on a protein level. Diluting AD-MSC conditioned media to varying levels of conditioning cells/mL produced a dose-dependent effect (dose 1:0=500,000 conditioning cells/mL) (C). Data are presented as mean±SEM with *, significant difference at p<0.05. n=4 was used per group in all experiments.