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. Author manuscript; available in PMC: 2015 Feb 19.
Published in final edited form as: Biochem J. 2012 May 1;443(3):789–798. doi: 10.1042/BJ20112062

Figure 4. CK2 overexpression can destabilize Nkx3.2 protein.

Figure 4

(A) Nkx3.2-Flag encoding plasmid was co-transfected with HA-PKCα into ATDC5 cells. After 48 h of transfection, cells were harvested and total cell lysates were analyzed by western blotting.

(B) ATDC5 cells were transfected with Nkx3.2-HA expression vector for 48 h, and then cells were treated with increasing doses (0, 0.5, 1, 2, and 3 μM) of Inonomycin for the final 6 h. Total cell lysates were then analyzed by western blotting.

(C) Nkx3.2-Flag encoding expression vector was co-transfected with Myc-TAK1 expression plasmid into ATDC5 cells, and, after 48 h of transfection, cells were harvested and total cell lysates were analyzed by western blotting.

(D) ATDC5 cells were transfected with Nkx3.2-Flag expression plasmid in the presence of HA-CK1α, δ, or ε expression vectors. At 48 h post-transfection, cells were processed for western blot analyses.

(E) Nkx3.2-Myc or Flag-Runx2 expression constructs were transfected into ATDC5 cells in the absence or presence of HA-CK2α and HA-CK2β expression plasmids. After 48 h of transfection, cells were harvested and total cell lysates were analyzed by western blotting.

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