FIG 2.

Bioinformatic analyses of PA2783 (Mep72). (A) Genomic context of bamI (PA2782) and mep72. Schematic locations of the primers used to confirm the operonic organization of bamI and mep72 are indicated. (B) cDNA (+RT) from biofilms was used as a template for PCR with the primers indicated in panel A to amplify the intergenic region between bamI and mep72. A reaction without reverse transcriptase (−RT) was carried out as a control. (C) Predicted domain structure of the metzincin Mep72. Numbers correspond to the amino acid residues comprising each domain and the predicted secretion signal sequence (Sig seq). The full-length protein is 599 residues long.