FIG 3.

mep72 expression in biofilms and planktonic cultures. (A) qRT-PCR analysis of mep72 and bamI transcript levels in cells sampled from planktonic exponential-phase (PE) and 1-day-old continuous-flow biofilms (1dB). Similar results were obtained in comparisons of 1dB biofilm-grown cells with planktonic stationary-phase cultures. The error bars represent standard deviations from 3 replicates. (B) Western blot analysis of Mep72 expression and secretion in PE, planktonic stationary-phase (PS), and continuous-flow biofilms of the wild-type (PAO1) and the mep72 mutant.