TABLE 1.

Summary of the identified protein spots modulated in the biofilm-grown culture secretome compared with their activity in the planktonic culture secretome

Function and PA no.	Master no.	Gene name	Gene product	Level of abundancea	Modulated ratiob (biofilm/PS)
Transport of small molecules
PA0301	1022	spuE	Polyamine transport protein	↑	19.8
	1033			↑	10.0
	971			↑	13.6
PA4687	996	hitA	Ferric iron-binding periplasmic protein HitA	↑	17.5
PA2592	1019	potF5	Probable periplasmic spermidine/putrescine-binding protein	↑	33.9
PA1493	960	cysP	Sulfate-binding protein of ABC transporter	↑	6.4
PA0888	1469	aotJ	Arginine/ornithine binding protein AotJ	↑	17.9
	1480			↑	14.1
PA1342	1155		Probable lysine/arginine/ornithine-binding protein	↑	9.9
Known virulence factors
PA0423	1765	pasP	P. aeruginosa small protease PASP	↑	5.2
PA1871	1687	lasA	LasA precursor	↑	16.5
PA4175	663	prpL	PvdS-regulated endoprotease, protease IV	↑	39.1
PA1706	1404	pcrV	Type III secretion protein PcrV	↑	17.6
PA0852	1170	cbpD	Chitin-binding protein CbpD precursor	↑	8.0
	1844			↓	37.9
	1830			↓	85.3
Unclassified hypothetical proteins
PA5033	1300		Hypothetical protein PA5033	↑	15.5
PA2783	1313	mep72	Hypothetical protein PA2783	↑	18.1
Central intermediary metabolism:	1721	ppa	Inorganic pyrophosphatase	↓	3.9
PA4031	1713			↓	3.4

^a↑ and ↓ indicate whether the spot was more or less abundant, respectively, in the biofilm secretome than in the planktonic secretome.

^bModulated ratio represents the fold change in the biofilm secretome compared to that in the planktonic secretome.