FIG 3.

algR and algZ genes are cotranscribed in both the nonmucoid and mucoid backgrounds. (A) Diagram showing the relative location and orientation of argH (PA5263), algZ (PA5262), and algR (PA5261) with the locations of the transcriptional start sites (bent arrows) ZT2, ZT1, RT2, and RT1. Primers (black arrows) and amplicons (solid lines) utilized for reverse transcriptase reactions are indicated. (B) Representative agarose gel with amplification of cDNA generated from the PAO1 mucB strain (lanes 1 to 4), PAO1 (lanes 5 to 8), or PAO1 genomic template control (lanes 9 to 12). The algZ and algR products correspond to the expression of the respective genes; algZR amplifies both in a single transcript, and algZR-C controls for chromosomal contamination.