FIG 4.

algZR and hemCD genes are cotranscribed in PAO1. (A) Amplification of algR and progressively further downstream genes hemC, hemD, PA5258, and PA5257 from the no-RT control, PAO1 chromosomal DNA, and cDNA resolved in an agarose gel. (B) Diagram showing the locations of oligonucleotide sets used in panels A (oligonucleotide sets 1 to 4) and C (oligonucleotide sets 5 to 9), which use the following oligonucleotides: set 1, algRGSF7 and hemCNR; set 2, algRGSF7 and hemD-R2; set 3, algRGSF7 and PA5258-R2; set 4, algRGSF7 and PA5257-R2. Arrows indicate primers, and solid lines indicate amplicons. (C) RT-qPCR of fimU, hemC, and PA5257 in the PAO1 and PAO1 ΔalgZ mutant backgrounds normalized to rpoD expression.