FIG 3.

Nucleotide sequence of the region upstream of bglA showing the putative promoter elements. The A residue at +1 indicates the transcription start site (TSS) corresponding to genome coordinate 3041661 reported in RegulonDB (http://regulondb.ccg.unam.mx/index.jsp). The triangle shows the position of the 4-nucleotide insertion within the spacer region between the predicted −10 and −35 elements in the Esc⁺ and Sal⁺ mutants. The insertion results in a new putative −35 element, improving the suboptimal spacer region of the wild-type promoter. The putative bglA translational start site is shown in green.