TABLE 1.
List of strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| E. coli strains | ||
| RV | F− lacX74 thi bglR0 bglG+ bglF+ bglB+ (Arb− Sal−) | 17 |
| RV Esc+ | RV bglR (Arb+ Sal+ Esc+) | This work |
| MM1 | RV tnaA::Tn10 (80% linkage to the wild-type bgl operon) | 17 |
| AE328 | MM1 bglR::IS1 (Arb+ Sal+) | 17 |
| SD-2.12 | RV bglR::IS1 ΔbglB (Arb+ Esc− Sal−) | 7 |
| SD-3.12 | SD-2.12 (Arb+ Esc+ Sal+) | 7 |
| SD-3.12 ΔascB | SD-3.12 ascB::kan (Arb+ Esc− Sal−) | 7 |
| SD-3.12 ΔascB Esc+ | SD-3.12 ΔascB bglAE1 (Arb+ Esc+ Sal−) | This work |
| SD-3.12 ΔascB Sal+ | SD-3.12 ΔascB bglAS1 (Arb+ Esc+ Sal+) | This work |
| SD-3.12 ΔascB ΔbglA | SD-3.12 ΔascB bglA677::Tn10 (Arb− Esc− Sal−) | This work |
| Plasmids | ||
| pACDH | Tetr, Plac promoter, pACYC184 origin | 18 |
| pACDH-bglAWT | Wild-type bglA under Plac promoter in pACDH | This work |
| pACDH-bglAEsc+ | bglA from Esc+ mutant under Plac promoter in pACDH | This work |
| pACDH-bglASal+ | bglA from Sal+ mutant under Plac promoter in pACDH | This work |
| pACDH-bglAT583G | bglAT583G under Plac promoter in pACDH | This work |