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. 2014 Oct 22;16(21):5656–5659. doi: 10.1021/ol502731p

Figure 2.

Figure 2

(a) Wild-type strain SNC-109 and rifampicin-resistant mutant strains SNC-109-M3 grown on media. Multiple concentrations of rifampicin on the different disks. (b) Mutation analysis of the β-subunit of bacterial RNA polymerase (RNAP) of SNC-109-M1, -M2, and -M3. RNAP gene segment for the wild-type and three mutants. Amino acid numbering based on S. coelicolor A3. (c) LC–MS traces of SNC-109 wild type and mutant strain SNC-109-M3. Compounds 1–3 can only be observed in the mutant strain (UV detection at λ 280 nm). The major peak in wild type strain SNC-109 at 7.5 min has a significantly different UV profile (Figure S5, Supporting Information).