Fig 2. Effect of drinking citrus fruit juice on cell proliferation in injured femoral artery after cuff placement.

Representative photos of injured femoral artery in cross-sections after PCNA staining (A) (200x and 400x magnification) and PCNA labeling index in intima (B) and media (C). Higher magnified photos described as squares in Figure A. Cuff placement around the femoral artery was performed, and PCNA was detected as described in Methods. Cell proliferation was measured as the ratio of PCNA-positive nuclei to total nuclei in the femoral artery 7 days after cuff placement. CU; Citrus unshiu juice, CI; Citrus iyo juice. a; adventitia, m; media, i; intima. Values are mean ± SEM (n = 6 for Cuff (-), n = 4 for other groups). *p<0.05, **p<0.01 vs. Cuff (+) Control, †p<0.05 vs. administration of juice of different citrus fruit at same %.