Figure 3.
Effect of α-T and δ-T on AR reporter activity in CWR-22Rv1/AR cells. CWR-22Rv1/AR cells were seeded at a density of 0.5 × 105 cells/mL of medium for 24 h. The medium was then changed to RPMI supplemented with 1% FBS, and the cells were treated with vehicle (control), with DHT (10 nM) alone or in combination with α-T or δ-T (20, 50, or 100 μM) for 24 h. The luciferase activity and protein concentration of the CWR-22Rv1/AR cells were measured. Each value represents the mean ± SE from three separate experiments. Differences for the relative luciferase activities between the α-T-treated group and the δ-T-treated group were analyzed by ANOVA with the Tukey-Kramer Multiple Comparison Test **p < 0.01, ***p < 0.001.