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. 1973 Apr;70(4):1127–1131. doi: 10.1073/pnas.70.4.1127

Isolation of Messenger RNA Coding for Mouse Heavy-Chain Immunoglobulin

R H Stevens 1, A R Williamson 1
PMCID: PMC433440  PMID: 4515608

Abstract

A method for purification of the mRNA coding for heavy-chain protein of a mouse G2a myeloma (5563) is reported. Complete myeloma protein (H2L2) specifically binds to the heavy-chain mRNA, and the resultant RNA-protein complex is precipitated with antiserum against 5563 myeloma protein. Cytoplasmic RNA isolated by this method showed two bands of 6.0 × 105 and 3.1 × 105 molecular weight when analyzed by acrylamide gel electrophoresis. The RNA in the bands contained 1.5% and 8% A-rich regions, respectively. The RNA in each of the bands contained sequences for heavy-chain protein when translated in Xenopus laevis oocytes.

Keywords: myeloma, Xenopus laevis oocyte injection, antiserum, acrylamide gel electrophoresis

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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