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. 2015 Mar 1;28(2):93–100. doi: 10.1089/vim.2014.0058

FIG. 6.

FIG. 6.

Levels of intracellular IL-12p35 from CD4+CD25+ Treg and CD4+CD25− T-cells. CD4+CD25+ Treg and CD4+CD25− T-cells were both activated with anti-CD3, anti-CD28, and rhIL-2 for 0, 3, 5, and 7 days. Intracellular cytokine IL-12p35 was detected by flow cytometry. The levels of IL-12p35 became higher, accompanied by a longer activation time in CD4+CD25+ Treg cells (F=141.14, p<0.05). However, lower levels of IL-12p35 were detected in unactivated and activated CD4+CD25− T-cells. Levels of intracellular cytokine IL-12p35 in CD4+CD25+ Tregs were higher than in CD4+CD25− Tregs at 0, 3, 5, and 7 days respectively (Table 4 and Fig. 5). CD4+CD25+ Treg and CD4+CD25− T-cells were acquired through MACS from fresh PBMCs.