FIG. 2.

(A–C) Confocal micrographs of whole scaffold surface (cell seeding side) after CellTracker™ Green staining. Original magnification x4 of representative areas of interest superimposed in the upper right corner. (D–F) SEM images representing cell adhesion characteristics on the scaffold surface. White bar represents 200 μm. (A, D) PCL scaffold, (B, E) NSP-PCL scaffold, and (C, F) HT-PCL scaffold. All images from (A–F) are acquired from scaffolds from day 21. (G–J) Hoechst staining of cell nuclei on histology sections from each scaffold on day 1(G), 7(H), 14(I), and 21(J). Each picture represents a section through the entire height of the scaffold. Image was color inverted and transformed from black background with blue fluorescence, to white background with grayscale fluorescence. An unedited color image of the fluoroscopy is available as Supplementary Data (Supplementary Fig. S2).