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. 2015 Feb 13;10(2):e0117594. doi: 10.1371/journal.pone.0117594

Fig 1. Confirmation of mutations in msr genes.

Fig 1

Primers from the regions flanking the site of the antibiotic-resistance cassette were used in the PCR. A larger PCR product was observed when genomic DNA from the mutant (even-numbered lanes) was used compared to when wild-type genomic DNA was used (odd-numbered lanes) as a template because of the insertion of a larger antibiotic-resistance cassette. Primers P9 and P10 were used to verify mutation in msrA1 (Lanes 1 & 2), P11 and P12 for mutation in msrA2 (Lanes 3 & 4), P13 and P14 for mutation in msrA3 (Lanes 5 & 6), P15 and P16 for mutation in msrB (Lanes 7 & 8), and P17 and P18 for mutation in msrA1-B (Lanes 9 & 10). Lane M—DNA ladder.