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. 2015 Feb 13;10(2):e0118336. doi: 10.1371/journal.pone.0118336

Fig 3. The MEK/Erk pathway is indispensable for both FAT4 gene repression and Cofilin-mediated actin depolymerization by Src.

Fig 3

A. Western blotting for phosphorylated and total Erk1/2 (P-Erk1/2 and Erk1/2, respectively) in MCF-10A v-Src:ER cells. Serum-starved cells (16 h) were cultured in the presence or absence of the MEK inhibitor U0126 for 1 h (30 μM) and then treated with 1 μM TAM for 1 h. B. Staining for F-actin (Phalloidin) and nuclei (DAPI) in TAM-treated cells (24 h) after pretreatment with 30 μM U0126 for 1 h. White bars, 50 μm. C. Western blotting for the indicated proteins in TAM-treated cells after pretreatment with U0126. D. RT-qPCR analyses of FAT4 mRNA expression levels in TAM-treated cells after pretreatment with U0126 (mean ± SD, n = 3). E. RT-qPCR analyses of FAT4 mRNA expression levels in MDA-MB-231 cells treated with 20 μM U0126 for 24 h after pretreatment with control or FAT4 siRNA (siControl and siFAT4, respectively, 20 nM) (mean ± SD, n = 3). F. WST-1 Assay in U0126-treated MDA-MB-231 cells (48 h) after pretreatment with siFAT4 for 24 h (mean ± SD, n = 5, * indicates P < 0.05).