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. 2015 Feb 13;10(2):e0118336. doi: 10.1371/journal.pone.0118336

Fig 6. FAT4 negatively regulates the activity of the Hippo effector YAP/TAZ.

Fig 6

A. RT-qPCR analyses of FAT4 and CTGF mRNA levels in MCF-10A cells transfected with control or FAT4 siRNA (siControl and siFAT4, respectively, 30 nM) for 48 h (mean ± SD, n = 3). B. Immunofluorescence images of YAP/TAZ in MCF-10A cells transfected with siFAT4 for 48 h. The nuclei were stained with DAPI. White bars, 50 μm. C. RT-qPCR analysis of CTGF mRNA expression levels in 24 h TAM-treated MCF-10A v-Src:ER cells (mean ± SD, n = 3, ** indicates P < 0.01). D. Immunofluorescence images of YAP/TAZ in TAM-treated cells (24 h). The nuclei were stained with DAPI. White bars, 50 μm. E. Staining for F-actin (Phalloidin) and nuclei (DAPI) in cells transfected with siFAT4 for 48 h. White bars, 50 μm. F. Western blotting for the indicated proteins in 48 h siFAT4-treated cells.