Fig 6. Validation of Stat92E as a key regulator during Ras^V12; scrib ^-/- oncogenesis.

(A-C) Confocal images of eye-antennal imaginal discs, clones are indicated in green and DAPI in blue. (A) Wild type disc from a 5 day old larva with MARCM clones expressing GFP (B) An eye-antennal tumor taken from an 8 day old larva with the Ras^V12; scrib ^-/- clones. (C) An eye-antennal disc from an 8 day old larva with the Ras^V12; Stat92E^85c9, scrib ^-/- clones, showing partial rescue of the tumor phenotype. (D) Opening of predicted Stat92E enhancers from Ras^V12 ; scrib ^-/- tumors vs wild type (x-axis), compared to the opening of these enhancers when ectopically activating the JAK/STAT signaling pathway vs wild type (y-axis). (E) Predicted Stat92E enhancer region inside an intron of Imp, the enhancer is significantly more active in the Upd eye discs compared to wild type (logFC 1.28 padj 0.005) and even more so in the Ras^V12; scrib ^-/- tumors compared to wild type (logFC 3.44 padj 1e-33). (F) Heatmap showing the expression level of 28 genes [42] that have at least one activated enhancer predicted to be Stat92E responsive (Fig. 6A).