Fig 1. Isolation of a full-length TRPM3 cDNA from mouse retina by RT-PCR.

Four segments of TRPM3 were amplified from retina cDNA. The primer’s exon and orientation (sense vs antisense) are indicated. The 5’ and 3’ untranslated regions are shown in light grey, the coding region in dark, and splice variation (see text) in white. Restriction enzyme sites used for sequential cloning of overlap extension products are indicated by diamonds. The location of the BamHI-XhoI cDNA fragment encoding the antigen used to raise an anti-TRPM3 antiserum is shown.