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Determination of α-dioxygenase activity of Ppα-DOX. (A) Mass-spectral ions (m/z) recorded on Ppα-DOX-derived 2-hydroxy fatty acids (methyl ester/trimethylsilyl ether derivatives) and fatty aldehydes (O-methyloxime derivatives). (B) Fatty acid substrate specificity of oxygenation by Ppα-DOX (mean +/− SE of n = 3–5 measurements).
