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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Biotechnol Bioeng. 2013 Nov 14;111(3):475–484. doi: 10.1002/bit.25127

Figure 2.

Figure 2

A purified FAP-ZZ affinity reagent is determined functional in-vitro. A: Schematic of fusion protein in expression plasmid: N-terminal hexa-histidine (6-His) tag is followed by a FAP domain connected via a small linker to the ZZ peptide. B: Fluorimetry data using 500 nM (MG-FAP)-ZZ protein and 1 uMMG-2p fluorogen in PBS. C: SDS–PAGE gel image of IgG-chromatography with purified (MG-FAP)-ZZ reagent